Lab #4: bacterial growth media & cultures lab this is the main page for the media & culture laboratory topic of an 8-week introductory college microbiology class the following links will take you to the virtual microbiology classroom documents that you will need to prepare for lab. About the bacteria other than morphology and arrangement the gram stain is a differential stain commonly used in the microbiology laboratory that differentiates bacteria on the basis of their cell wall. Study flashcards on microbiology: lab exercise 2-smear preparation, bacterial morphology at cramcom quickly memorize the terms, phrases and much more cramcom makes it easy to get the grade you want.
Microbiology unknown lab report samantha moellenhoff microbiology, fall 2012 introduction with the constant debate in society about microorganisms, it is important to distinguish their identities. That most bacteria are transparent and cannot be seen through the microscope the gram stain is a type of differential stain that allows a (micro)biologist to water was placed on a microscope slide and bacteria from unknown number 20 were also placed on the slide then the slide had to be air dried. Bacteria morphology lab report this report will detail how a similar project was performed in order to identify two unknown micobes using the techniques that were learnt during the course of the semester. A bacterial species, since it reveals both the morphology and the gram reaction of the bacteria in this exercise, both gram-positive and gram-negative rods and cocci of.
Lab 3: morphological characteristics of bacteria protocols for endospore stain, capsule stain, motility stab and wet mount introduction bacteria are characterized by the presence or absence of a number of different. Bacterial colony morphology bacteria grow on solid media as colonies a colony is defined as a visible mass of microorganisms all originating from a single. Bacterial morphology part1mp4 this feature is not available right now please try again later. Report your final abundance of cultivable bacteria to the spreadsheet on the central lab computer or the blackboard for your final lab report, discuss the differences in cultivable bacteria among the sampled. In this lab you will be innoculating plates and observing bacterial growth microscopes can then be used to identify specific bacteria this lab may take several days, keep all data and observations in a separate notebook to be compiled and organized into a final lab report.
A more reliable method to identify cell morphology would be to use special stains to identify specific parts of a microbe like endospores, which is usually present in gram positive bacteria the method use to stain endospores is called the schaeffer-fulton1 method, where malachite green is used to stain the endospores while safranin is a. Therefore, the number of bacterial cells in the original sample is determined by multiplying the number of colonies on a dilution plate by the corresponding dilution factor for example, if you counted 200 colonies on the 10-4 plate, 200 x 10,000 = 2,000,000 colonies or 2 x 106 viable cells/ml were present in the original sample. How to write an unknown lab report in microbiology general unknown reports in microbiology are written in scientific format scientific writing is written. Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives the procedure is based on the ability of microorganisms to retain color of the stains used during the gram stain reaction.
The first test performed on the gram positive bacteria was the nitrate test which turned red after adding reagents giving a positive result meaning the bacteria reduced nitrate into nitrite or something further. Lab exercise #2 - bacterial media & culture 2 the medium that we use most often is designated tsy (tryptic soy agar) and it is a complex nutrient medium which supports the growth of a wide variety of microbes. Unknown lab report unknown number 109 tyler wolfangel april 29, 2014 bio 203-604 introduction the study of microbiology requires not only an academic understanding of the microscopic world but also a practical understanding of lab techniques and procedures used to identify, control, and manipulate microorganisms.
identification of unknown report abstract: the purpose of this lab was to determine the identity of an unknown bacteria slant culture the unknown was identified using differential tests to determine its metabolic and morphology characteristics. After the morphology was observed and recorded, a gram stain was performed on each, this procedure is found in the laboratory manual the two microorganisms were identified one was a gram-positive bacteria, the other was a gram-negative bacteria. Viewing prepared slides of bacterial morphology disinfecting the workspace, using live cultures and cells such as a cheek smear, teeth plaque and yeast were examined via wet mount staining was done to all of these cultures with a gram stain and as well as a congo red stain.
That most bacteria are transparent and cannot be seen through the microscope the gram stain is a type of differential stain that allows a (micro)biologist to identify the differences between organisms and/or differences within the same. The mordant is the substance that set dyes the iodine in gram stain fixed the crystal violet to bacterial cell wall of the gram positive bacteria when gram-positive bacteria are treated with ethanol , the alcohol is thought to shrink the pores of thick peptidoglogycan. General unknown reports in microbiology are written in scientific format scientific writing is written differently from other types of writing the results of the exercise or experiment are what are being showcased, not the writing the purpose of scientific writing is not to entertain, but to.